Transcription profiling of 8-day-old Podocin-Cre, Z/EG double transgenic mice glomerulus and brain

ABSTRACT: Mouse glomeruli and brain capillary fragments were prepared. Podocytes were separated from isolated glomeruli from 8-day-old Podocin-Cre, Z/EG double transgenic mice as follows: Isolated glomeruli were incubated with trypsin solution containing 0.2 % trypsin-EDTA (Sigma-Aldrich), 100ug/ml Heparin and 100U/ml DNase I in PBS for 25 min at 37 degree, with mixing by pipetting every 5 min. The trypsin was inactivated with soybeans trypsin inhibitor (Sigma-Aldrich) and the cell suspension sieved through a 30 um pore size filter (BD bioscience, Franklin Lakes, NJ). Cells were collected by centrifugation at 200 x g for 5 min at 4 degree and resuspended in 1ml PBS supplemented with 0.1 % BSA. To separate GFP-expressing (GFP+) and GFP-negative (GFP-) cells, glomerular cell were sorted using a FACSVa

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Mus musculus

SUBMITTER: Christer Betsholtz 

PROVIDER: E-MEXP-492 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress