Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of thymus from myasthenia gravis patients

ABSTRACT: Identification of deregulated genes in the thymus of myasthenia gravis patients' subgroup.

INSTRUMENT(S): 428 [Affymetrix]

ORGANISM(S): Homo sapiens

DISEASE(S): myasthenia gravis (seropositive patient with low thymic hyperplasia)

SUBMITTER: Géraldine Clairac

PROVIDER: E-MEXP-518 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Microarrays reveal distinct gene signatures in the thymus of seropositive and seronegative myasthenia gravis patients and the role of CC chemokine ligand 21 in thymic hyperplasia.

Le Panse Rozen R Cizeron-Clairac Géraldine G Bismuth Jacky J Berrih-Aknin Sonia S

Journal of immunology (Baltimore, Md. : 1950) 20061201 11

Myasthenia gravis (MG) is an autoimmune disease mainly caused by antiacetylcholine receptor autoantibodies (seropositive (SP) disease) or by Abs against unknown autoantigenic target(s) (seronegative (SN) disease). Thymectomy is usually beneficial although thymic hyperplasia with ectopic germinal centers is mainly observed in SP MG. To understand the role of thymus in the disease process, we compared the thymic transcriptome of non-MG adults to those of SP patients with a low or high degree of hy ...[more]

PMID: 17114458

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Project description:The antenno-maxilary complex (AMC) forms the chemosensory system of the Drosophila larva and is involved in gustatory and olfactory perception. We have previously shown that a mutant allele of the homeodomain transcription factor Prospero (prosVoila1, V1), presents several developmental defects including abnormal growth and altered taste responses. In addition, many neural tracts connecting the AMC to the central nervous system (CNS) were affected. Our earlier reports on larval AMC did not argue in favour of a role of pros in cell fate decision, but strongly suggested that pros could be involved in the control of other aspect of neuronal development. In order to identify these functions, we used microarray analysis of larval AMC and CNS tissue isolated from the wild type, and three other previously characterised prospero alleles, including the V1 mutant, considered as a null allele for the AMC. A total of 17 samples were first analysed with hierarchical clustering. To determine those genes affected by loss of pros function, we calculated a discriminating score reflecting the differential expression between V1 mutant and other pros alleles. We identified a total of 64 genes in the AMC. Additional manual annotation using all the computed information on the attributed role of these genes in the Drosophila larvae nervous system, enabled us to identify a first functional category of potential Prospero target genes known to be involved in neurite outgrowth, synaptic transmission and more specifically in neuronal connectivity remodelling. The second category of genes found to be differentially expressed between the null mutant AMC and the other alleles concerned the development of the sensory organs and more particularly the larval olfactory system. Surprisingly, a third category emerged from our analyses and suggests an association of pros with the genes that regulate autophagy, growth and insulin pathways. Interestingly, EGFR and Notch pathways were represented in all of these three functional categories. We now propose that Pros could perform all of these different functions through the modulation of these two antagonistic and synergic pathways. The current data contribute to the clarification of the Prospero function in the larval AMC and show that pros regulates different function in larvae as compared to those controlled by this gene in embryos. In the future, the possible mechanism by which Pros could achieve its function in the AMC and the possible involvement of EGFR and Notch pathway will be explored in detail Gene expression was measured in two different tissues (Brain and AMC) of prospero mutants flies (V1, V13, V14, V24). 3 biological replicates were performed for V1, V13 and V24 AMC and V14 Brain; 2 biological replicates for V14 AMC and V13 Brain.

Dataset Information

Transcription profiling of thymus from myasthenia gravis patients

Publications

Microarrays reveal distinct gene signatures in the thymus of seropositive and seronegative myasthenia gravis patients and the role of CC chemokine ligand 21 in thymic hyperplasia.

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