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Transcription profiling of individual bovine nuclear transfer blastocysts

ABSTRACT: Individual zona free in vitro grown bovine day 7 blastocysts were compared to stage and quality matched nuclear transfer derived blastocysts (fibroblast donor cells).

INSTRUMENT(S): GenePix 4000A [Axon Instruments]

ORGANISM(S): Bos taurus

SUBMITTER: Peter Pfeffer

PROVIDER: E-MEXP-556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiling of individual bovine nuclear transfer blastocysts.

Somers Joanna J Smith Craig C Donnison Martyn M Wells David N DN Henderson Harold H McLeay Lance L Pfeffer P L PL

Reproduction (Cambridge, England) 20060601 6

During somatic cell nuclear transfer the gene expression profile of the donor cell has to be changed or reprogrammed extensively to reflect that of a normal embryo. In this study we focused on the switching on of embryonic genes by screening with a microarray consisting of 5000 independent cDNA isolates derived from a bovine blastocyst library which we constructed for this purpose. Expression profiling was performed using linearly amplified RNA from individual day 7 nuclear transfer (NT) and gen ...[more]

PMID: 16735546

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