Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis Col-0 plants with wild type, arr1-1 knock-out, ARR1 over-expressor and ARR1-SRDX fusion protein over-expressor genotypes treated with 6-Benzyladenine for different lengths of time


ABSTRACT: Arabidopsis thaliana Col-0 plants and three other genotypes (ARR1 overexpressor, arr1-1 knockout, overexpressor of ARR1-SRDX fusion protein) were grown in liquid media (1/2 MS, 1 g/L sucrose, 0.5 g/L MES, pH 5.7) in a Percival AR-66L growth chamber at 24 oC, 16:8 h day:night cycle, and 100 µE light intensity until growth stage 1.0. Plants were then treated with 5 µM 6-Benzyladenine for 0, 15, and 120 min, harvested and frozen in liquid nitrogen for RNA extraction and subsequent processing for microarray hybridization.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Wolfram Brenner 

PROVIDER: E-MEXP-689 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

In planta analysis of a cis-regulatory cytokinin response motif in Arabidopsis and identification of a novel enhancer sequence.

Ramireddy Eswarayya E   Brenner Wolfram G WG   Pfeifer Andreas A   Heyl Alexander A   Schmülling Thomas T  

Plant & cell physiology 20130424 7


The phytohormone cytokinin plays a key role in regulating plant growth and development, and is involved in numerous physiological responses to environmental changes. The type-B response regulators, which regulate the transcription of cytokinin response genes, are a part of the cytokinin signaling system. Arabidopsis thaliana encodes 11 type-B response regulators (type-B ARRs), and some of them were shown to bind in vitro to the core cytokinin response motif (CRM) 5'-(A/G)GAT(T/C)-3' or, in the c  ...[more]

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