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Transcription profiling of mouse glioma cell line grown in two types of media to investigate cell de-differentiation

ABSTRACT: Comparison of gene expression profiles of the GL261 cell line (a murine glioma model) grown in duplicate in two different types of media. AC samples where grown in DMEM supplemented by 20% FBS, 5 U/ml pen/strep and 4 mM L-glutamine. NS samples were grown in DMEM/F12 (50/50) supplemented with 2 U/ml pen/strep, 1 ug/ml fungizone, 1x B27, 20 ng/ml bFGF, 20 ng/ml EGF, 20 ng/ml LIF and 5 ug/ml heparin. We have reason to believe the NS media enhances cell de-differentiation.

ORGANISM(S): Mus musculus

SUBMITTER: Blanca Suarez-Merino 

PROVIDER: E-MEXP-718 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neurospheres enriched in cancer stem-like cells are highly effective in eliciting a dendritic cell-mediated immune response against malignant gliomas.

Pellegatta Serena S   Poliani Pietro Luigi PL   Corno Daniela D   Menghi Francesca F   Ghielmetti Francesco F   Suarez-Merino Blanca B   Caldera Valentina V   Nava Sara S   Ravanini Maria M   Facchetti Fabio F   Bruzzone Maria Grazia MG   Finocchiaro Gaetano G  

Cancer research 20061101 21

Cancer stem-like cells (CSC) could be a novel target for cancer therapy, including dendritic cell (DC) immunotherapy. To address this, we developed experiments aimed at DC targeting of neurospheres (NS) from GL261 glioma cells because neurospheres can be enriched in CSC. We obtained murine neurospheres by growing GL261 cells in epidermal growth factor/basic fibroblast growth factor without serum. GL261-NS recapitulated important features of glioblastoma CSC and expressed higher levels of radial  ...[more]

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