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Transcription profiling time series of Sinorhizobium meliloti in response to an osmotic upshift elicited by salt or sucrose

ABSTRACT: Time course of the S. meliloti response to an osmotic upshift elicited by salt (300mM or 400mM) or sucrose (500mM or 700mM)

INSTRUMENT(S): ScanArray 4000 [PerkinElmer]

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: Rebeca Pérez-Arnedo 

PROVIDER: E-MEXP-785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti.

Domínguez-Ferreras Ana A   Pérez-Arnedo Rebeca R   Becker Anke A   Olivares José J   Soto María J MJ   Sanjuán Juan J  

Journal of bacteriology 20060817 21

In this work, DNA microarrays were used to investigate genome-wide transcriptional responses of Sinorhizobium meliloti to a sudden increase in external osmolarity elicited by addition of either NaCl or sucrose to exponentially growing cultures. A time course of the response within the first 4 h after the osmotic shock was established. We found that there was a general redundancy in the differentially expressed genes after NaCl or sucrose addition. Both kinds of stress resulted in induction of a  ...[more]

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