Transcription profiling of young bees raised in colonies with a queen, with queen mandibular pheromone (QMP), or without a queen.
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ABSTRACT: This experiment was designed to identify gene expression differences in young bees raised in colonies with a queen, with queen mandibular pheromone (QMP), or without a queen. The source colony was split into three colonies, allocating roughly equal quantities of adult bees, brood, and pollen and nectar stores. One colony retained the original queen (QR), one was left queenless (QL), and the third was given a strip that contained 10 Qeq (QMP+), a dose shown to mimic a live queen (20). The three colonies were transferred to a different apiary > 2 mi away so they would not return to the site of their natal hive. Prior to the colony split, bees (N ~1500) were collected 0-36 h after eclosion, marked on the dorsal thorax with a paint dot (Testors Paint), and ~500 were placed in each of the three colonies. Two days later, the marked bees were collected (N = 100). Bees were collected into liquid nitrogen, and heads were stored as above.
ORGANISM(S): Apis mellifera
SUBMITTER: Charles Whitfield
PROVIDER: E-MEXP-80 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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