Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of wild type and BOLITA over-expressing Arabidopisis leaves

ABSTRACT: Comparison of BOLITA mutant leaves (gain-of-function mutant) vs. wild type leaves, grown in the same conditions. The BOLITA (BOL) gene (At1g24590), an AP2/ERF transcription factor, was characterized with the help of an activation tag mutant and overexpression lines in Arabidopsis and tobacco. The leaf size of plants overexpressing BOL was smaller than wild type plants due to a reduction in both cell size and cell number. Moreover, severe overexpressors showed ectopic callus formation in roots. Accordingly, global gene expression analysis using the overexpression mutant reflected the alterations in cell proliferation, differentiation and growth through expression changes in RBR, CYCD, and TCP genes, as well as genes involved in cell expansion (i.e. expansins and the actin remodeling factor ADF5). Furthermore, the expression of hormone signaling (i.e. auxin and cytokinin), biosynthesis (i.e. ethylene and jasmonic acid) and regulatory genes was found to be perturbed in bol-D mutant leaves. The gene is expressed at the shoot apical meristem, and more intensely at leaf primordia. It is also expressed at very young leaves, and flower buds. The gene is closely related to DORNROSCHEN.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Stefan de Folter

PROVIDER: E-MEXP-809 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways.

Marsch-Martinez Nayelli N Greco Raffaella R Becker Jörg D JD Dixit Shital S Bergervoet Jan H W JH Karaba Aarati A de Folter Stefan S Pereira Andy A

Plant molecular biology 20061012 6

The BOLITA (BOL) gene, an AP2/ERF transcription factor, was characterized with the help of an activation tag mutant and overexpression lines in Arabidopsis and tobacco. The leaf size of plants overexpressing BOL was smaller than wild type plants due to a reduction in both cell size and cell number. Moreover, severe overexpressors showed ectopic callus formation in roots. Accordingly, global gene expression analysis using the overexpression mutant reflected the alterations in cell proliferation, ...[more]

PMID: 17096212

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Dataset Information

Transcription profiling of wild type and BOLITA over-expressing Arabidopisis leaves

Publications

BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways.

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