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Transcription profiling of diploid and tetraploid yeast cell cultures

ABSTRACT: Diploid and tetraploid budding yeast cell cultures were grown in YPD, at 30C, to O.D. approx. 0.5.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Zuzana Storchova

PROVIDER: E-MEXP-822 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide genetic analysis of polyploidy in yeast.

Storchová Zuzana Z Breneman Amanda A Cande Jessica J Dunn Joshua J Burbank Kendra K O'Toole Eileen E Pellman David D

Nature 20061001 7111

Polyploidy, increased sets of chromosomes, occurs during development, cellular stress, disease and evolution. Despite its prevalence, little is known about the physiological alterations that accompany polyploidy. We previously described 'ploidy-specific lethality', where a gene deletion that is not lethal in haploid or diploid budding yeast causes lethality in triploids or tetraploids. Here we report a genome-wide screen to identify ploidy-specific lethal functions. Only 39 out of 3,740 mutation ...[more]

PMID: 17024086

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