Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse lung from wildtype and glucocorticoid recepter knockouts to investigate investigate the role of glucocorticoids in fetal lung development

ABSTRACT: The aim of this experiment is to investigate the role of glucocorticoids in fetal lung development in more detail.

INSTRUMENT(S): GenePix 4000A [Axon Instruments]

ORGANISM(S): Mus musculus

SUBMITTER: Kheng Tan

PROVIDER: E-MEXP-861 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Identification of glucocorticoid-regulated genes that control cell proliferation during murine respiratory development.

Bird Anthony D AD Tan Kheng H KH Olsson P Fredrik PF Zieba Malgorzata M Flecknoe Sharon J SJ Liddicoat Douglas R DR Mollard Richard R Hooper Stuart B SB Cole Timothy J TJ

The Journal of physiology 20070927 Pt 1

Glucocorticoids play a vital role in fetal respiratory development and act via the intracellular glucocorticoid receptor (GR) to regulate transcription of key target genes. GR-null mice die at birth due to respiratory dysfunction associated with hypercellularity and atelectasis. To identify events associated with this lung phenotype we examined perinatal cellular proliferation rates and apoptotic indices. We demonstrate that compared to wild-type controls, day 18.5 postcoitum (p.c.) GR-null mous ...[more]

PMID: 17901120

Similar Datasets

Project description:Phenotypic plasticity, the ability of one genotype to express different phenotypes in response to changing environmental conditions, is one of the most common phenomena characterising the living world and is not only relevant for the ecology but also for the evolution of species. Daphnia, the waterflea, is a textbook example for predator induced phenotypic plastic defences including changes in life-history, behaviour and morphology. However, the analysis of molecular mechanisms underlying these inducible defences is still in its early stages. We exposed Daphnia magna to chemical cues of the predator Triops cancriformis to identify key processes underlying plastic defensive trait formation. D. magna is known to develop an array of morphological changes in the presence of T. cancriformis including changes of carapace morphology and cuticle hardening. To get a more comprehensive idea of this phenomenon, we studied four different genotypes originating from habitats with different predation history, reaching from predator-free to temporary habitats containing T. cancriformis. We analysed the morphologies as well as proteomes of predator-exposed and control animals. Three genotypes showed morphological changes when the predator was present. Using a high-throughput proteomics approach, we found 294 proteins which were significantly altered in their abundance after predator exposure in a general or genotype dependant manner. Proteins connected to genotype dependant responses were related to the cuticle, protein synthesis and calcium binding whereas the yolk protein vitellogenin increased in abundance in all genotypes, indicating their involvement in a more general response. Furthermore, genotype dependant responses at the proteome level correlated well with local adaptation to Triops predation. Altogether, our study provides new insights concerning genotype dependant and general molecular processes involved in predator-induced phenotypic plasticity in D. magna.

Dataset Information

Transcription profiling of mouse lung from wildtype and glucocorticoid recepter knockouts to investigate investigate the role of glucocorticoids in fetal lung development

Publications

Identification of glucocorticoid-regulated genes that control cell proliferation during murine respiratory development.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets