Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human THP-1 cells in response to treatment with lipo-polysaccharide for 1 or 4 hours

ABSTRACT: Effect of LPS in a time dependent manner (1hr or 4hr treatment v untreated) on the THP-1 cell using a focused microarray containing 465 putative or known NF-kappaB regulated genes.

INSTRUMENT(S): arrayWoRx Multi-Format Reader [Applied Precision]

ORGANISM(S): Homo sapiens

SUBMITTER: Neil. D Perkins

PROVIDER: E-MEXP-868 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional profiling of the LPS induced NF-kappaB response in macrophages.

Sharif Omar O Bolshakov Viacheslav N VN Raines Stephanie S Newham Peter P Perkins Neil D ND

BMC immunology 20070112

<h4>Background</h4>Exposure of macrophages to bacterial products such as lipopolysaccharide (LPS) results in activation of the NF-kappaB transcription factor, which orchestrates a gene expression programme that underpins the macrophage-dependent immune response. These changes include the induction or repression of a wide range of genes that regulate inflammation, cell proliferation, migration and cell survival. This process is tightly regulated and loss of control is associated with conditions s ...[more]

PMID: 17222336

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Project description:Phenotypic plasticity, the ability of one genotype to express different phenotypes in response to changing environmental conditions, is one of the most common phenomena characterising the living world and is not only relevant for the ecology but also for the evolution of species. Daphnia, the waterflea, is a textbook example for predator induced phenotypic plastic defences including changes in life-history, behaviour and morphology. However, the analysis of molecular mechanisms underlying these inducible defences is still in its early stages.<br><br>We exposed Daphnia magna to chemical cues of the predator Triops cancriformis to identify key processes underlying plastic defensive trait formation. D. magna is known to develop an array of morphological changes in the presence of T. cancriformis including changes of carapace morphology and cuticle hardening. To get a more comprehensive idea of this phenomenon, we studied four different genotypes originating from habitats with different predation history, reaching from predator-free to temporary habitats containing T. cancriformis.<br><br>We analysed the morphologies as well as proteomes of predator-exposed and control animals. Three genotypes showed morphological changes when the predator was present. Using a high-throughput proteomics approach, we found 294 proteins which were significantly altered in their abundance after predator exposure in a general or genotype dependant manner. Proteins connected to genotype dependant responses were related to the cuticle, protein synthesis and calcium binding whereas the yolk protein vitellogenin increased in abundance in all genotypes, indicating their involvement in a more general response. Furthermore, genotype dependant responses at the proteome level correlated well with local adaptation to Triops predation.<br><br>Altogether, our study provides new insights concerning genotype dependant and general molecular processes involved in predator-induced phenotypic plasticity in D. magna.

Dataset Information

Transcription profiling of human THP-1 cells in response to treatment with lipo-polysaccharide for 1 or 4 hours

Publications

Transcriptional profiling of the LPS induced NF-kappaB response in macrophages.

Similar Datasets

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