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Methylation profiling of normal and cancerous human breast samples

ABSTRACT: To assess changes in DNA methylation state associated with breast cancer we analyzed DNA from normal and cancerous breast specimens on CpG islands microarray using methylation-sensitive enzyme McrBC for target preparation. Cy3 was used for McrBC cut sample, Cy5 was used for reference Mock sample.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Homo sapiens

DISEASE(S): phyllodes tumor

SUBMITTER: Walter Klimecki

PROVIDER: E-MEXP-881 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epigenetic inactivation of the HOXA gene cluster in breast cancer.

Novak Petr P Jensen Taylor T Oshiro Marc M MM Wozniak Ryan J RJ Nouzova Marcella M Watts George S GS Klimecki Walter T WT Kim Christina C Futscher Bernard W BW

Cancer research 20061106 22

Using an integrated approach of epigenomic scanning and gene expression profiling, we found aberrant methylation and epigenetic silencing of a small neighborhood of contiguous genes-the HOXA gene cluster in human breast cancer. The observed transcriptional repression was localized to approximately 100 kb of the HOXA gene cluster and did not extend to genes located upstream or downstream of the cluster. Bisulfite sequencing, chromatin immunoprecipitation, and quantitative reverse transcription-PC ...[more]

PMID: 17090521

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