Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunoprecipitation of Arabidopsis seedlings to identify targets of TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1 and H3K27me3, H3K9me2 and H3K9me3

ABSTRACT: Chromatin immunoprecipitation and hybridization to a chromosome-wide DNA tiling array (ChIP-chip)was performed to identify the targets of the chromatin protein TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1. Epigenomic profiling was also carried out for three histone H3 modifications: H3K27me3, H3K9me2 and H3K9me3. Experiments were done using two independent biological replicates.

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Vincent Colot 

PROVIDER: E-MEXP-951 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Arabidopsis TFL2/LHP1 specifically associates with genes marked by trimethylation of histone H3 lysine 27.

Turck Franziska F   Roudier François F   Farrona Sara S   Martin-Magniette Marie-Laure ML   Guillaume Elodie E   Buisine Nicolas N   Gagnot Séverine S   Martienssen Robert A RA   Coupland George G   Colot Vincent V  

PLoS genetics 20070417 6

TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1 (TFL2/LHP1) is the only Arabidopsis protein with overall sequence similarity to the HETEROCHROMATIN PROTEIN 1 (HP1) family of metazoans and S. pombe. TFL2/LHP1 represses transcription of numerous genes, including the flowering-time genes FLOWERING LOCUS T (FT) and FLOWERING LOCUS C (FLC), as well as the floral organ identity genes AGAMOUS (AG) and APETALA 3 (AP3). These genes are also regulated by proteins of the Polycomb repressive complex 2 (PRC  ...[more]

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