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Transcription profiling of callus, petals and stamens from wild type and gmyb10 over-expressing gerbera plants in order to identify target genes for gmyb10 in the anthocyanin pathway

ABSTRACT: In the microarray experiment a comparison of different flower organs of transformed transgenic gerbera line to wild type gerbera was done. In the transgenic gerbera line the gene gmyb10 that regulates anthocyanin pathway is over-expressed. In this line anthocyanin pigmentation is altered compared to the wild type. In this experiment RNA from dark red callus, two stage of petal development and stamens of the transgenic lines were compared to RNA of wild type in order to identify target genes for gmyb10.

INSTRUMENT(S): ScanArray scanning hardware

ORGANISM(S): Gerbera hybrid cultivar

SUBMITTER: Roosa Laitinen

PROVIDER: E-MEXP-959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida.

Laitinen Roosa A E RA Ainasoja Miia M Broholm Suvi K SK Teeri Teemu H TH Elomaa Paula P

Journal of experimental botany 20080825 13

Genetic modification of the flavonoid pathway has been used to produce novel colours and colour patterns in ornamental plants as well as to modify the nutritional and pharmaceutical properties of food crops. It has been suggested that co-ordinate control of multiple steps of the pathway with the help of regulatory genes would lead to a more predictable control of metabolic flux. Regulation of anthocyanin biosynthesis has been studied in a common ornamental plant, Gerbera hybrida (Asteraceae). An ...[more]

PMID: 18725377

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