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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of yeast grown in SD media with addition of either methionine or hydrogen peroxide

ABSTRACT: Yeast cells were grown up in SD media containing all required amino acids. Each strain set was performed in triplicate. One set had no changes, the second set had 1mM methionine supplenting the media for the duration of growth and the third set was exposed to 0.5mM hydrogen peroxide for 15 minutes prior to harvesting

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andy Hayes

PROVIDER: E-MEXP-998 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gcn4 is required for the response to peroxide stress in the yeast Saccharomyces cerevisiae.

Mascarenhas Claire C Edwards-Ingram Laura C LC Zeef Leo L Shenton Daniel D Ashe Mark P MP Grant Chris M CM

Molecular biology of the cell 20080416 7

An oxidative stress occurs when reactive oxygen species overwhelm the cellular antioxidant defenses. We have examined the regulation of protein synthesis in Saccharomyces cerevisiae in response to oxidative stress induced by exposure to hydroperoxides (hydrogen peroxide, and cumene hydroperoxide), a thiol oxidant (diamide), and a heavy metal (cadmium). Examination of translational activity indicates that these oxidants inhibit translation at the initiation and postinitiation phases. Inhibition o ...[more]

PMID: 18417611

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