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RNA-seq of brain organoids from human NKX2.1(GFP/w) hESCs treated with patterning molecules

ABSTRACT: There are on-going efforts to steer brain organoid development toward distinct regional identities by supplying cues in defined culture conditions. Here we incubated developing organoids with a number of patterning molecules at different concentrations to assess their effects on establishing brain region identities.

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Homo sapiens

SUBMITTER: Gray Camp

PROVIDER: E-MTAB-10037 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Resolving organoid brain region identities by mapping single-cell genomic data to reference atlases.

Fleck Jonas Simon JS Sanchís-Calleja Fátima F He Zhisong Z Santel Malgorzata M Boyle Michael James MJ Camp J Gray JG Treutlein Barbara B

Cell stem cell 20210311 6

Self-organizing tissues resembling brain structures generated from human stem cells offer exciting possibilities to study human brain development, disease, and evolution. These 3D models are complex and can contain cells at various stages of differentiation from different brain regions. Single-cell genomic methods provide powerful approaches to explore cell composition, differentiation trajectories, and genetic perturbations in brain organoid systems. However, it remains a major challenge to und ...[more]

PMID: 33711282

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RNA-seq of brain organoids from human NKX2.1(GFP/w) hESCs treated with patterning molecules

Project description:RNA-seq of brain organoids from human NKX2.1(GFP/w) hESCs treated with patterning molecules

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Neuronal tangential migration from Nkx2.1-positive hypothalamus.

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