Transcription profiling of human peripheral blood monocytes from a patient with a heterozygous deletion of the CARMA1 gene cultured with anti-CD3, anti-CD3 and anti-CD28 or lipopolysaccharide
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ABSTRACT: PBMCs were isolated from whole blood taken from one patient and one healthy control. Cells were cultured in media (RPMI) alone, with 1µg/ml anti-CD3, with 1µg/ml anti-CD3 & 1µg/ml anti-CD28 or with 0.5ng/ml LPS. Cells were harvested after 24 or 48 hours, RNA isolated using Trizol, purified using RNeasy columns and labelled using Illumina TotalPrep RNA Amplificiation kit (Applied Biosystems) before hybridisation to Illumina HumanRef-8 arrays.
ORGANISM(S): Homo sapiens
SUBMITTER: Simon Wadell
PROVIDER: E-MTAB-101 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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