Allele-resolved scRNA-seq of primary mouse fibroblasts using Smart-seq3
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ABSTRACT: Allele-sensitive RNA sequencing of single-cells can be used to infer the kinetics of transcriptional bursts in eukaryotic cells. Here, we used the Smart-seq3 protocol to prepare libraries from two 384-well plates of primary mouse fibroblasts. The fibroblasts were derived from tail explants of a male adult mouse (F1 offspring of C57 x CAST cross). The samples were sequenced to high depth using MGI's DNBSEQ G400RS platform using paired-end 100 bp reads.
INSTRUMENT(S): DNBSEQ-G400
ORGANISM(S): Mus musculus
SUBMITTER: Christoph Ziegenhain
PROVIDER: E-MTAB-10148 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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