Unknown,Transcriptomics,Genomics,Proteomics

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Allele-resolved scRNA-seq of primary mouse fibroblasts using Smart-seq3


ABSTRACT: Allele-sensitive RNA sequencing of single-cells can be used to infer the kinetics of transcriptional bursts in eukaryotic cells. Here, we used the Smart-seq3 protocol to prepare libraries from two 384-well plates of primary mouse fibroblasts. The fibroblasts were derived from tail explants of a male adult mouse (F1 offspring of C57 x CAST cross). The samples were sequenced to high depth using MGI's DNBSEQ G400RS platform using paired-end 100 bp reads.

INSTRUMENT(S): DNBSEQ-G400

ORGANISM(S): Mus musculus

SUBMITTER: Christoph Ziegenhain 

PROVIDER: E-MTAB-10148 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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