Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

RNA-seq comparing WT to HDAC3 and Dax1 knock-out clones in cell-sorted populations in 2iLIF and SL(RA) differentiation conditions

ABSTRACT: In order to determine the transcriptomic profiles of PrE-induced cells following HDAC3 or Dax1 knock-out in SL(RA) differentiation conditions, RNA-seq was performed on Gata6+ or Nanog+ sort-selected cells. Profiles are contrasted with WT cells in the same differentiation conditions as well as to mock-sorted populations of the three genotypes in 2iLIF conditions.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Daniel Olivieri 

PROVIDER: E-MTAB-10150 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Mouse embryonic stem cells (mESCs) are biased toward producing embryonic rather than extraembryonic endoderm fates. Here, we identify the mechanism of this barrier and report that the histone deacetylase Hdac3 and the transcriptional corepressor Dax1 cooperatively limit the lineage repertoire of mESCs by silencing an enhancer of the extraembryonic endoderm-specifying transcription factor Gata6. This restriction is opposed by the pluripotency transcription factors Nr5a2 and Esrrb, which promote c  ...[more]

Similar Datasets

Transcriptomics RNA-seq comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones in 2iLIF and SL differentiation conditions
2021-03-15 | E-MTAB-9447 | biostudies-arrayexpress
Transcriptomics ATACseq of TNG-A derived mESCs comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones
2021-03-15 | E-MTAB-9453 | biostudies-arrayexpress
Transcriptomics Single-cell RNA-seq comparing WT, HDAC3 and Dax1 knock-out clones in SL(RA) differentiation conditions
2021-03-15 | E-MTAB-10147 | biostudies-arrayexpress
Transcriptomics RNA-seq comparing WT to HDAC3 knock-out clones in naïve mESCs, as well as SL and EpiLC differentiation conditions at two distinct time points (1 and 2 days)
2021-03-14 | E-MTAB-9446 | biostudies-arrayexpress
Transcriptomics RNA-seq for wild-type, Zbtb2, Zfp369 and Ubn2 knock-out clones of mESCs in 2iLIF and SerumLif differentiation conditions.
2021-07-07 | E-MTAB-9798 | biostudies-arrayexpress
Transcriptomics ChIPseq for histone marks in WT and Hdac3 knock-out clones of TNG-A derived mESCs in 2iLIF and SL differentiation conditions
2021-03-15 | E-MTAB-9450 | biostudies-arrayexpress
Transcriptomics RNA-seq for wild-type and Zbtb2 knock-out clones of mESCs in 2iLIF, EpiLC and SerumLIF conditions.
2021-07-07 | E-MTAB-9797 | biostudies-arrayexpress
Genomics RNA-seq comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones in 2iLIF and SL differentiation conditions
| PRJEB39928 | ENA
Transcriptomics Gene expression profile after Dax1 deletion in ES cells
2015-05-13 | GSE61152 | GEO
Transcriptomics Pluripotency-state-dependent Role of Dax1 in Embryonic Stem Cells Self-renewal
2021-07-21 | GSE168423 | GEO