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Dynamic in vivo labelling reveals the continuous trafficking of stem-like TCF1+ CD8 T cells through tumours

ABSTRACT: Single cell RNA seq of photo converted tumour infiltrating cells (TIL) at 24h and 72h post-conversion.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Syed Murtuza Baker

PROVIDER: E-MTAB-10176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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In vivo labeling reveals continuous trafficking of TCF-1+ T cells between tumor and lymphoid tissue.

Li Zhi Z Tuong Zewen K ZK Dean Isaac I Willis Claire C Gaspal Fabrina F Fiancette Rémi R Idris Suaad S Kennedy Bethany B Ferdinand John R JR Peñalver Ana A Cabantous Mia M Murtuza Baker Syed S Fry Jeremy W JW Carlesso Gianluca G Hammond Scott A SA Dovedi Simon J SJ Hepworth Matthew R MR Clatworthy Menna R MR Withers David R DR

The Journal of experimental medicine 20220426 6

Improving the efficacy of immune checkpoint therapies will require a better understanding of how immune cells are recruited and sustained in tumors. Here, we used the photoconversion of the tumor immune cell compartment to identify newly entering lymphocytes, determine how they change over time, and investigate their egress from the tumor. Combining single-cell transcriptomics and flow cytometry, we found that while a diverse mix of CD8 T cell subsets enter the tumor, all CD8 T cells retained wi ...[more]

PMID: 35472220

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