Project description:Candida albicans lab strain SC5314 was daily passaged in YPD broth supplemented with fluconazole. Some fluconazole-resistant and some fluconazole-tolerant adaptors were sequenced.
Project description:Candida albicans lab strain SC53114 was used to obtain fluconazole-tolerant adaptors. 53 adaptors as well as the parent strain were sequenced.
Project description:Candida albicans isolate YJB-T490 was spread on YPD plates supplemented with fluconazole. The plates were incubated at 37C. 54 adaptors were sequenced.
Project description:Candida haemulonii wild type strain TJ180 were expsoured to 64 and 128 μg/ml fluconazole. 14 resistant mutants as well as the parent were sequenced.
Project description:Candida auris clade III isolate B12039 was spread on YPD plate supplemented with 64 µg/ml fluconazole. Randomly 45 adaptors were chosen for further analysis. We did sequencing of them as as well as the parent.
Project description:Candida auris clade III isolate B12039 was spread on YPD plate supplemented with 128 µg/ml fluconazole. Randomly 39 adaptors were chosen for further analysis. We did sequencing of them as as well as the parent.
Project description:Candida albicans lab strain SC5314 was spread on YPD plates supplemented with echinocandins (caspofungin, anidulafungin, micafungin, rezafungin). The adaptors were tested for tolrance to fluconazole. Randomly 43 tolerant adaptors, as well as the parent SC5314, were sequenced.
Project description:In Cryptococcus neoformans, H99 is the wild type. TJ1854 is the chromosome 4 disomy strain dervied from H99. In this study, approximately 1 million cells of TJ1854 were spread on YPD plate supplemented with 32ug/ml fluconazole. Randomly 27 adaptors (TJ3249-TJ3275) were chosen. These adaptors were sequenced.
Project description:In Cryptococcus neoformans, H99 is the wild type. TJ1843 is a strains with segmental disomy of chromosome 11, which is derived from H99. In this study, approximately 1 million cells of TJ1843 were spread on YPD plate supplemented with 32 ug/ml fluconazole. Randomly 27 adaptors (TJ3305 and TJ3331) were chosen. These adaptors were sequenced.
Project description:Candida albicans isolate YJB-T490 was spread on YPD plates supplemented with fluconazole. The plates were incubated at 30˚C. 54 adaptors as well as the parent were sequenced.