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Intestinal ulcers from biopsy-wounded wild-type and Bmp4-overexpressing mice

ABSTRACT: We performed RNAseq on ulcers and distant normal tissue from biopsy-wounded wild-type and Bmp4-overexpressing (Vil1-Cre;Rosa26-Bmp4) mice to investigate differences in gene expression between tissue states and genotypes. Biopsy wounding was performed by taking three biopsies from well-separated areas in the colon wall using forceps. Small tissue pieces were excised from the ulcers and distant normal colon tissue and collected for RNA sequencing.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Gabriel Valbuena

PROVIDER: E-MTAB-10238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bone Morphogenetic Protein Pathway Antagonism by Grem1 Regulates Epithelial Cell Fate in Intestinal Regeneration.

Koppens Martijn A J MAJ Davis Hayley H Valbuena Gabriel N GN Mulholland Eoghan J EJ Nasreddin Nadia N Colombe Mathilde M Antanaviciute Agne A Biswas Sujata S Friedrich Matthias M Lee Lennard L Wang Lai Mun LM Koelzer Viktor H VH East James E JE Simmons Alison A Winton Douglas J DJ Leedham Simon J SJ

Gastroenterology 20210402 1

<h4>Background & aims</h4>In homeostasis, intestinal cell fate is controlled by balanced gradients of morphogen signaling. The bone morphogenetic protein (BMP) pathway has a physiological, prodifferentiation role, predominantly inferred through previous experimental pathway inactivation. Intestinal regeneration is underpinned by dedifferentiation and cell plasticity, but the signaling pathways that regulate this adaptive reprogramming are not well understood. We assessed the BMP signaling landsc ...[more]

PMID: 33819486

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