NGS data of Nicotiana benthamiana 16c plants after CRISPR/Cas9 gene editing

ABSTRACT: Provided data came from a detailed study on Nicotiana benthamiana 16c plants where we use Tobacco Rattle Virus (TRV) as a molecular switch to change the chromatin state of a reporter gene (P35S::GFP) from an actively transcribed to a transcriptionally silenced state. Our approach enables us to interrogate different chromatin states of the same locus with the same set of CRISPR/Cas9 genome editing reagents and systematically describe the effect of chromatin state on the frequency and type of mutations induced at various Cas9 targets in a huge set of independently edited cells.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Nicotiana benthamiana

SUBMITTER: Adéla Přibylová 

PROVIDER: E-MTAB-10256 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress