Transformics experiment on BALB/c 3T3 cells treated with B(a)P 0.02 µg/ml to investigate BaP mechanisms of carcinogenic action

ABSTRACT: This analysis utilised the \"Transformics Assay\" and evaluated the effect of cell exposure to 0.02 μg/ml of Benzo(a)pyrene, the lowest transforming concentration, using the BALB / c 3T3 A31-1-1 cell line. The Transformic assay is an integrated approach combining the Cell Transformation Assay (CTA), with the high throughput technology of transcriptomics. The CTA is a widely used in vitro assay that has been designed to evaluate the effects of chemicals on the growth of specific cell types and their potential progression through a transformation process from being normal cells to fully malignant cells. It has the potential to detect both genotoxic and some non-genotoxic carcinogens and shows a good correlation with rodent bioassay data. However lack of understanding of the mechanistic ba

INSTRUMENT(S): Agilent Microarray Scanner (mod. G2505C)

ORGANISM(S): Mus musculus

SUBMITTER: Maria Grazia Mascolo 

PROVIDER: E-MTAB-10405 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress