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RNA-seq of primary human macrophages uninfected or infected with Yersinia enterocolitica strains

ABSTRACT: Pathogenic bacteria Yersinia enterocolitica injects virulence plasmid-encoded effectors through the type three secretion system into macrophages to modulate gene expression. At this point there are no comprehensive gene expression analysis in primary human macrophages analyzing the effect of virulence plasmid-encoded factors on transcription. For this primary human macrophages were infected for 1.5 h and 6 h with mock, WAC (virulence plasmid-cured strain) or WA314 (wild type) and samples were subjected to RNA-seq. The effect of effector protein YopP on gene expression in macrophages was analyzed using a wild type strain lacking YopP.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Indra Bekere

PROVIDER: E-MTAB-10473 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-seq of primary human macrophages infected with Yersinia enterocolitica strains

Project description:Pathogenic bacteria Yersinia enterocolitica injects virulence plasmid-encoded effectors through the type three secretion system into macrophages to modulate gene expression. Here we analyzed the effect on gene expression in primary human macrophages of Y. enterocolitica strains lacking effector YopP (1.5 h infection) or effectors YopP and YopM (1.5 h or 6 h infection) simultaneously using RNA-seq. This is part of a larger sequencing experiment for which other samples can be found in EMBL-EBI (www.ebi.ac.uk/arrayexpress) under accession number E-MTAB-10473 and European Nucleotide Archive (ENA) at http://www.ebi.ac.uk/ena/data/view/PRJEB10086.

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