Unknown,Transcriptomics,Genomics,Proteomics

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Activation of normal dermal fibroblasts by normal and cancer epithelial cells. Tissue cultures.

ABSTRACT: We model processes of wound healing and tumor growth, by studying the effects of normal and cancer epithelial cells on normal fibroblasts and their reciprocal effect on normal keratinocytes in vitro. We find strong parallels between the two processes and compare our observations with transcriptional analysis of 24 clinical samples of squamous cell carcinoma. This dataset contains the tissue culture samples, for the clinical samples, see E-MTAB-1065. Normal human fibroblasts (HF) were cocultured with either normal HK or transformed epithelial cells (HaCaT and FaDu) and their expression profile was compared to the expression profile of HF cultured alone.

ORGANISM(S): Homo sapiens

SUBMITTER: Michal Kolar 

PROVIDER: E-MTAB-1064 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses.

Kolář Michal M   Szabo Pavol P   Dvořánková Barbora B   Lacina Lukáš L   Gabius Hans-Joachim HJ   Strnad Hynek H   Sáchová Jana J   Vlček Cestmír C   Plzák Jan J   Chovanec Martin M   Cada Zdeněk Z   Betka Jan J   Betka Jan J   Fík Zdeněk Z   Pačes Jan J   Kovářová Hana H   Motlík Jan J   Jarkovská Karla K   Smetana Karel K  

Biology of the cell 20121201 12

<h4>Background information</h4>Considering an analogy between wound healing and tumour progression, we studied chemokine and cytokine transcription and expression in normal fibroblasts by co-culture and in situ.<h4>Results</h4>Whole-genome transcriptome profiling revealed strong upregulation for the interleukin (IL)-6, IL-8 and the chemokine CXCL-1 in in vitro co-cultures of normal fibroblasts with either normal or malignant epithelial cells compared to fibroblast cultures. The same ILs/chemokin  ...[more]

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