Project description:Pull-down of endogenous N-terminally TAP-tagged ERBP1 (Tb927.10.14150). RNA of unbound and eluted samples was purified, rRNA of unbound sample was depleted, and RNA was analysed by RNA-Seq.
Project description:DRBD7 interacting mRNAs were identified using a DRBD7-TAP fusion protein for protein pulldown. To study enrichment, the flow-through was also subjected to RNAseq.
Project description:Affinity purification of RBSR1 and RBSR2 endogenously tagged with TAP at the C terminal. A cell line with inducible G-TAP was used as negative control. 3 biological replicates were used for RBSR1 and RBSR2 and 3 technical replicates for GFP
Project description:RBP9-TAP was expressed in bloodstream T. brucei and bound mRNA was pulled down using IgG beads and eluted via TEV-protease cleavage. rRNA was depleted using RNAseH and rRNA hybridizing oligos.
Project description:T. brucei CPSF73 endogenously TAP-tagged at the C-terminal was affinity purified after treating the cells for 15 min with AN7973 at 10x EC50. Three technical replicates were used. 3 technical replicates of untreated cells served as controls. Inducible GFP-TAP with and without treatment served as additional controls