Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

36 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-seq of Drosophila melanogaster plasmatocytes against various histone modifications

ABSTRACT: To identify the presence of different chromatin states in homogeneous primary cells, Drosophila melanogaster plasmatocytes were isolated from 3rd instar larvae and subjected to cross-linked ChIP-seq using antibodies against a range of H3 histone modifications and PolII.

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Robert Streeck

PROVIDER: E-MTAB-10707 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

ChIP-seq of Drosophila melanogaster OregonR plasmatocytes at 6h post septic injury

Project description:Drosophila 3rd instar larvae were subjected to septic injury. After 6h plasmatocytes were isolated, fixed and subjected to ChIP-seq.

2022-05-31 | E-MTAB-10760 | biostudies-arrayexpress

RNA-seq of resting plasmatocytes from Drosophila melanogaster third instar larvae

Project description:Resting plasmatocytes were isolated from 3rd instar larvae and subjected to RNA-seq as comparison to ChIP-seq data

2022-05-31 | E-MTAB-10759 | biostudies-arrayexpress

RNA-seq of Hml-SwitchGal4 induced pathway activation in Drosophila plasmatocytes

Project description:Drosophila carrying Hml-SwitchGal4 were crossed to different uas-constructs (uas-eGFP, uas-PGRP-LC, uas-hop, uas-tkvQD). 3rd instar larvae were either kept as control or SwitchGal4 was activated by hormone feeding for 24h. Afterwards plasmatocytes were extracted and subjected to RNA-seq.

2022-05-31 | E-MTAB-10764 | biostudies-arrayexpress

RNA-seq of plasmatocytes after septic injury

Project description:OrR drosophila 3rd instar larvae were subjected to septic injury with a mixture of E.coli and S.aureus at 3h, 6h and 18h. Plasmatocytes were isolated afterwards and subjected to RNA-seq

2022-05-31 | E-MTAB-10761 | biostudies-arrayexpress

RNA-seq of plasmatocytes after septic injury with trxG-RNAis

Project description:Drosophila plasmatocyes expressed control or trxG RNAis under the plasmatocyte specific Hml promotor. 6h prior to plasmatocyte collection animals were split into control and septic injury groups and treated accordingly. Afterwards plasmatocytes were isolated and polyA RNA was subjected to RNA-seq.

2022-05-31 | E-MTAB-10780 | biostudies-arrayexpress

RNA-seq of Drosophila plasmatocyte mosaics which lack PRC2/H3K27me3

Project description:Genetic mosaics were induced in 3rd instar larvae by heatshock to generate H3K27R mutants, Su(z)12 mutants or E(z) mutants

2022-05-31 | E-MTAB-10762 | biostudies-arrayexpress

PROTEOMIC RESPONSE OF EARLY JUVENILE PACIFIC OYSTERS TO TEMPERATURE

Project description:The shellfish aquaculture industry provides a sustainable food source and jobs for a growing population. Oysters are the primary aquaculture species produced in the United States and account for a significant portion of seafood exports. Shellfish hatcheries have been experiencing frequent mass mortality events over the last couple of decades that occur approximately 10-14 days after oyster settlement. Settlement is a process that shellfish such as oysters undergo in which they transform from a free-swimming pelagic larvae to a sessile juvenile oyster. In order for this energy-intensive process to be successful, the oyster has to undergo behavioral and morphological changes. This is a vulnerable period in the oyster life cycle and conditions need to be such that they aren’t creating added stress. However, due to the oysters’ vulnerability, this is often a time when bacterial infections can occur, which when occurring with environmental conditions that are unfavorable, can prove to be fatal. In order to help oysters survive this process, scientists at the Taylor Shellfish Hatchery in Quilcene, WA has experimented with altering abiotic and biotic factors such as algal diet densities, pH, water flow rate, among others. At this hatchery, Pacific oysters are typically reared at 23˚C, however preliminary research results have suggested that oysters may have a higher survival rate when held at 29˚C during the settlement period. This pilot experiment attempts to identify differences in protein expression between oyster seed held at 23˚C and 29˚C during the settlement period using novel proteomic technology. Our proteomic results, paired with survival data, suggest that holding oyster seed at 29˚C during the settlement period results in higher survival rates.

2022-03-03 | PXD015434 | Pride

Peptide of Trichinella spiralis infective larval extract that harnesses growth of human hepatoma cells

Project description:This project aimed to explore novel anticancer therapeutics from products of parasite since several data related to benefits from the T. spiralis infection have been documented. We validated antitumor activity of T. spiralis infective larval extract and extricate the parasite-derived-antitumor peptide. We found that larval extract exerted antitumor activity to three types of carcinoma cells including hepatocellular carcinoma HepG2, ovarian cancer SK-OV-3, and lung adenocarcinoma A549. Interestingly, it displayed the most antitumor effect to HepG2 cells. Using proteomic and bioinformatic approaches, three putative anticancer peptides were identified from T. spiralis infective larval extract. One of these peptides showed a dose-dependent-anti-HepG2 effect by inducing ROS accumulation, leading to inhibition of the cell proliferation. Our data indicate potential application of the larval extract-derived antitumor peptide as a complementary agent for human hepatoma treatment and highlight a positive aspect of parasite apart from its deleterious effect.

2022-04-13 | PXD032081 | Pride

RNA-seq experiment of Drosophila melanogaster larvae reared on Aspergillus nidulans infested breeding substrate

Project description:Drosophila melanogaster larvae and filamentous fungi both utilise organic material. Here they compete for resources. Filamentous fungi can defend themselves and their substrate from predation respectively competition by the production and excretion of secondary metabolites, including substances with antibiotic and insecticidal properties. To analyse the traits that enables D. melanogaster larvae to reduce the harmful effects of fungal secondary metabolites and to develop on fungal infested substrate we confronted larvae with a toxin-producing wild type of Aspergillus nidulans, with a toxin-production-impaired mutant strain of A. nidulans, and with sterigmatocystin, a highly toxic metabolite of A. nidulans. Early first instar larvae were transferred to breeding substrate inhabited by fungal colonies respectively inoculated with the purified mycotoxin or controls. After 3, 6, 12, and 24 hours of confrontation larvae were collected and samples prepared for whole transcriptome shotgun sequencing.

2017-12-01 | E-MTAB-5344 | biostudies-arrayexpress

RNA-seq of haemoless drosophila larvae compared to matching control animals

Project description:Drosophila melanogaster 3rd instar wandering larvae with a genetic construct for hemocyte ablation were collected along with genetically matched non-ablated animals, RNA was extracted from whole animals and used for RNA-seq.

2022-09-14 | E-MTAB-11095 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data