Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human LNCaP prostate cancer cell line to identify FOXP3 targets

ABSTRACT: As we clarified before, the FOXP3 gene is an X-linked tumor suppressor gene of both human and mouse. We also clarified that the ERBB2, SKP2 and p21 genes were transcriptionally under control of FOXP3 in human epithelial cells. In order to further clarify the FOXP3 down stream targets in human cancer cells, we conducted a microarray analysis of FOXP3-induced gene expression profiling. A human prostate cancer cell line, LNCaP, was transfected either with a FOXP3-eGFP expressing vector or an eGFP-expressing vector. After 48 hrs of cell culture, we isolated eGFP-positive LNCaP cells by FACS sorting, and then total RNA from those cells were extracted by Qiagen's RNeasy column and they were applied to Affymetrix Human U133 2.0 array according to the manufacture's protocol. We clarified as yet unknown FOXP3 target genes in human prostate epithelial cells by this analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Lizhong Wang 

PROVIDER: E-MTAB-108 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Despite clear epidemiological and genetic evidence for X-linked prostate cancer risk, all prostate cancer genes identified are autosomal. Here, we report somatic inactivating mutations and deletion of the X-linked FOXP3 gene residing at Xp11.23 in human prostate cancer. Lineage-specific ablation of FoxP3 in the mouse prostate epithelial cells leads to prostate hyperplasia and prostate intraepithelial neoplasia. In both normal and malignant prostate tissues, FOXP3 is both necessary and sufficient  ...[more]

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