MBD-Seq was applied to study the CpG methylation map of rhabdomyosarcoma cells after DiPRO1/ZNF555 inhibition
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ABSTRACT: The methylation enrichment was determined by MIRA-sequencing after MBD2-capture using the MethylCollector™ Ultra Kit (Active Motif) performed on DNA samples derived from rhabdomyosarcoma cells (RMS) TE671 cells. The cells expressing shRNA targeting ZNF555 (shZNF555) were compared to the control cells expressing non-targeting shRNA (shCtl). The signals of methylation-enriched DNA (ENR) were normalised to non-enriched input DNA (INP).
INSTRUMENT(S): Qubit, Covaris S220 (LGC Genomics GmbH), Bioanalyseur 2100 (Agilent), Bravo Platform (Agilent), Illumina NovaSeq 6000
ORGANISM(S): Homo sapiens
SUBMITTER: Iryna Pirozhkova
PROVIDER: E-MTAB-10825 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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