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ScRNA-seq of E9.5 para-aortic splanchnopleura from NCX1-null embryos and wild type controls

ABSTRACT: In order to study the effect of Ncx1 deletion on the HSC-supportive microenviromrent in the AGM niche, caudal parts from E9.5 (20-23 sp) Ncx1-/- and Ncx1+/+ littermate controls (4 embryos/group) were dissected and processed into single cell suspensions and dead/damaged cells were removed by magnetic separation. Resulting cell suspension from Ncx1-/- and Ncx1+/+ embryos loaded onto independent channels of a Chromium chip before single cell partitioning and barcoding on Chromium Controller (10x Genomics).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Giorgio Anselmi

PROVIDER: E-MTAB-10945 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition.

Azzoni Emanuele E Frontera Vincent V Anselmi Giorgio G Rode Christina C James Chela C Deltcheva Elitza M EM Demian Atanasiu S AS Brown John J Barone Cristiana C Patelli Arianna A Harman Joe R JR Nicholls Matthew M Conway Simon J SJ Morrissey Edward E Jacobsen Sten Eirik W SEW Sparrow Duncan B DB Harris Adrian L AL Enver Tariq T de Bruijn Marella F T R MFTR

Cell reports 20211201 11

Hematopoietic stem cells (HSCs) emerge during development from the vascular wall of the main embryonic arteries. The onset of circulation triggers several processes that provide critical external factors for HSC generation. Nevertheless, it is not fully understood how and when the onset of circulation affects HSC emergence. Here we show that in Ncx1<sup>-/-</sup> mouse embryos devoid of circulation the HSC lineage develops until the phenotypic pro-HSC stage. However, these cells reside in an abn ...[more]

PMID: 34910918

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