Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profiling of peripheral blood mononuclear cells before and after eradication of Hepatitis C virus by DAAs

ABSTRACT: Remarkable improvements in the treatment of HCV infection have been observed since the availability of HCV Direct-antiviral agents (DAAs). We evaluated by microarray the transcriptomic profile of circulating mononuclear cells from 8 chronic HCV patients at baseline and 12 weeks after the end of 4 weeks treatment with a combination of IFN-free DAA therapy.

ORGANISM(S): Homo sapiens

SUBMITTER: Paola Pontrelli

PROVIDER: E-MTAB-10952 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pre-Transplant Expression of CCR-2 in Kidney Transplant Recipients Is Associated With the Development of Delayed Graft Function.

Pontrelli Paola P Simone Simona S Rascio Federica F Pesce Francesco F Conserva Francesca F Infante Barbara B Castellano Giuseppe G Sallustio Fabio F Fiorentino Marco M Zaza Gianluigi G Gallone Anna A Battaglia Michele M Ditonno Pasquale P Stallone Giovanni G Gesualdo Loreto L Grandaliano Giuseppe G

Frontiers in immunology 20220316

<h4>Background</h4>Delayed graft function (DGF) leads to a reduced graft survival. Donors' features have been always considered as key pathogenic factors in this setting. The aim of our study was to evaluate the recipients' characteristics in the development of DGF.<h4>Methods</h4>We enrolled 932 kidney graft recipients from 466 donors; 226 recipients experienced DGF. In 290 donors, both recipients presented with early graft function (EGF, group A), in 50 both recipients experienced DGF (group B ...[more]

PMID: 35371047

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Project description:Chronic antibody-mediated rejection (CAMR) represents the main cause of kidney graft loss, but its pathogenesis is unclear. In order to uncover the molecular mechanisms underlying this condition, we characterized the molecular signature of circulating peripheral blood mononuclear cells and, separately, of CD4+ T lymphocytes isolated from CAMR patients compared to kidney transplant recipients with normal graft function and histology. In total peripheral lympho-monocytes, forty-five genes resulted differentially expressed between the two groups, most of them were up-regulated in CAMR and were involved in type I interferon signaling. In addition, in the same set of patients, 16 microRNAs resulted down-regulated in CAMR subjects compared to controls: 4 were predicted modulators of 6 mRNAs identified in the transcriptional analysis. In silico functional analysis supported the involvement of type I interferon signaling. To further confirm this hypothesis, we investigated the transcriptomic profiles of CD4+ T lymphocytes in an independent group of patients and we observed that the activation of type I interferon signaling was a specific hallmark of CAMR. In addition, in CAMR patients we detected a reduction of circulating BDCA2+ dendritic cells, the natural type I interferon- producing cells and their recruitment into the graft along with an increased expression of MXA, a type I interferon-induced protein, at tubulointerstitial and vascular level. In conclusion, our data suggest that type I interferon signaling may represent the molecular signature of CAMR. We performed microarray experiments in CD4+ T cells isolated from a small independent group of 5 patients included into the control-group and 5 included into the study group. The control group was represented by renal transplant recipients undergoing protocol graft biopsies, with normal renal function and histology, in the absence of circulating anti-HLA antibodies. Study group patients showed clinical and histological evidence of CAMR according to Banff 2011 criteria.

Dataset Information

Gene expression profiling of peripheral blood mononuclear cells before and after eradication of Hepatitis C virus by DAAs

Publications

Pre-Transplant Expression of CCR-2 in Kidney Transplant Recipients Is Associated With the Development of Delayed Graft Function.

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