Chip-seq to identify σE dependent genes in EtOH stressed and unstressed Streptomyces coelicolor A3(2)

ABSTRACT: Chip-seq experiment used to identify the binding sites of alternative σ factor σE in Streptomyces coelicolor. We access the binding sites with and without EtOH stress condition (see methods). To capture the binding of σE, the σE gene was tagged on genome by HA (Human Influenza hemagglutinin derived) epitope (TAC CCA TAC GAC GTC CCA GAC TAC GCT) on its C-terminus, wild type strain without tagged σE served as negative control.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Jiří Pospíšil 

PROVIDER: E-MTAB-10987 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress