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ScRNAseq libraries of pooled kidney samples from wild type (ZF1) versus CRISPR-targeted renin knockout (ZF2) zebrafish

ABSTRACT: Libraries were made to compare the transcriptome of renin lineage cells (RLCs) from wild-type versus ren knockout zebrafish kidneys. RLCs were FAC sorted from pooled kidneys of ren+/+ or ren-/- zebrafish, which carried ren:RFP and acta2:EGFP reporter genes, allowing the isolation of renin-expressing cells and smooth muscle cells.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Danio rerio

SUBMITTER: Linda Mullins

PROVIDER: E-MTAB-11079 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparative Studies of Renin-Null Zebrafish and Mice Provide New Functional Insights.

Hoffmann Scott S Mullins Linda L Rider Sebastien S Brown Cara C Buckley Charlotte B CB Assmus Adrienne A Li Ziwen Z Sierra Beltran Mariana M Henderson Neil N Del Pozo Jorge J De Goes Martini Alexandre A Sequeira-Lopez Maria Luisa S MLS Gomez R Ariel RA Mullins John J

Hypertension (Dallas, Tex. : 1979) 20220110 3

<h4>Background</h4>The renin-angiotensin system is highly conserved across vertebrates, including zebrafish, which possess orthologous genes coding for renin-angiotensin system proteins, and specialized mural cells of the kidney arterioles, capable of synthesising and secreting renin.<h4>Methods</h4>We generated zebrafish with CRISPR-Cas9-targeted knockout of renin (<i>ren</i><sup>-/-</sup>) to investigate renin function in a low blood pressure environment. We used single-cell (10×) RNA sequenci ...[more]

PMID: 35000430

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