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Single-cell immune repertoire sequencing of bone marrow plasma cells following infection and immunizations

ABSTRACT: We performed single-cell transcriptome and antibody repertoire sequencing of bone marrow plasma cells following protein (OVA and TNFR2) immunizations or infection with high dose LCMV clone 13.

INSTRUMENT(S): Illumina NovaSeq 6000, NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Alex Yermanos

PROVIDER: E-MTAB-11096 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Profiling the specificity of clonally expanded plasma cells during chronic viral infection by single-cell analysis.

Neumeier Daniel D Pedrioli Alessandro A Genovese Alessandro A Sandu Ioana I Ehling Roy R Hong Kai-Lin KL Papadopoulou Chrysa C Agrafiotis Andreas A Kuhn Raphael R Shlesinger Danielle D Robbiani Damiano D Han Jiami J Hauri Laura L Csepregi Lucia L Greiff Victor V Merkler Doron D Reddy Sai T ST Oxenius Annette A Yermanos Alexander A

European journal of immunology 20211123 2

Plasma cells and their secreted antibodies play a central role in the long-term protection against chronic viral infection. However, due to experimental limitations, a comprehensive description of linked genotypic, phenotypic, and antibody repertoire features of plasma cells (gene expression, clonal frequency, virus specificity, and affinity) has been challenging to obtain. To address this, we performed single-cell transcriptome and antibody repertoire sequencing of the murine BM plasma cell pop ...[more]

PMID: 34727578

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