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Transcription profiling by array of cerebella from Sbno2-/- mice

ABSTRACT: This study sought to investigate the role of Strawberry notch homolog 2 (Sbno2), an interleukin-6 (IL-6)-regulated gene whose function is largely unknown, in the central nervous system through the use of a novel mouse model with germline deletion of Sbno2 (Sbno2-/-). In particular, to understand its role in IL-6-mediated neurological disease, these mice were crossed with the GFAP-IL6 transgenic mice, which chronically produces IL-6 from in astrocytes at pathophysiological levels.

ORGANISM(S): Mus musculus

SUBMITTER: Taylor Syme

PROVIDER: E-MTAB-11169 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from mouse intestine: C57Bl/6 MTHFR+/- vs BALB/c MTHFR+/-

Project description:Previous studies in our laboratory have shown that low folate diet (control diet with 2mg folate/kg, low folate diet with 0.3mg folate/kg) can induce intestinal tumors in BALB/c mice. In addition, we reported that C57Bl/6J mice did not form tumors under the same conditions. We used microarrays to identify the genetic differences between BALB/c and C57Bl/6J mice that promote tumorigenesis in BALB/c mice. Two groups of mice will be used in the micro-array experiment. One group will consist of four BALB/c mice and the second group will consist of four C57Bl/6 mice. Both groups have mild MTHFR deficiency (Mthfr+/-) and will receive low folate diet (0.3 mg folate/kg) for one year. RNA samples will be extracted from duodenum, the first section of the small intestine where tumors were observed in BALB/c mice. Eight Affymetrix GeneChip Mouse Gene 1.0 ST Array Chips will be used in this experiment.

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Global genome expression analysis of lamina propria derived WT and Nlrp6-/- Ly6C-hi inflammatory monocytes

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