Ontology highlight
ABSTRACT:
ORGANISM(S): Pichia pastoris
SUBMITTER: Andrew Hesketh
PROVIDER: E-MTAB-1126 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Hesketh Andrew R AR Castrillo Juan I JI Sawyer Trevor T Archer David B DB Oliver Stephen G SG
Applied microbiology and biotechnology 20130911 22
Pichia pastoris is widely used as a host system for heterologous protein expression in both academia and industry. Production is typically accomplished by a fed-batch induction process that is known to have negative impacts on cell physiology that impose limits on both protein yields and quality. We have analysed recombinant protein production in chemostat cultures to understand the physiological responses associated with methanol-induced production of two human lysozyme variants with different ...[more]