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RNA-seq of small intestinal organoids treated with IL-22 for 0, 1 and 4 hours

ABSTRACT: The intestinal epithelium is our first line of defence against infections of the gut and the plasticity in cellular differentiation of the intestinal epithelium is an important part of this response. The changes in cellular composition is driven by immune cell derived cytokines. Here we investigate the response over time upon stimulation with 10 ng/mL of the cytokine Interleukin-22 (IL-22).

ORGANISM(S): Mus musculus

SUBMITTER: Naveen Parmar

PROVIDER: E-MTAB-11273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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BMP signaling in the intestinal epithelium drives a critical feedback loop to restrain IL-13-driven tuft cell hyperplasia.

Lindholm Håvard T HT Parmar Naveen N Drurey Claire C Campillo Poveda Marta M Vornewald Pia M PM Ostrop Jenny J Díez-Sanchez Alberto A Maizels Rick M RM Oudhoff Menno J MJ

Science immunology 20220513 71

The intestinal tract is a common site for various types of infections including viruses, bacteria, and helminths, each requiring specific modes of immune defense. The intestinal epithelium has a pivotal role in both immune initiation and effector stages, which are coordinated by lymphocyte cytokines such as IFNγ, IL-13, and IL-22. Here, we studied intestinal epithelial immune responses using organoid image analysis based on a convolutional neural network, transcriptomic analysis, and in vivo inf ...[more]

PMID: 35559665

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