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Analysis of mid-colon and mid-jejunum segments from C57BL/6J wild-type mice exposed to doxorubicin (DOX)

ABSTRACT: ADRs (adverse drug reactions) are one of the main reasons for treatment discontinuation or alterations in dose regimens in clinical settings. Chemotherapy-induced ADRs are common, especially gastrointestinal-induced ones. Within TransQST (transqst.org), one of the main goal is to improve the in vitro-in vivo translation in toxicological studies, as well as translation between non-clinical species (such as rat and mouse) and humans. This experimental setup therefore aimed to investigate the effects of DOX on mouse tissue derived from colon and jejunum biopsies. Experiments were conducted in a dose (5 and 10 mg/kg) and time series regimen (6, 24, 72 and 96h). Time-matched controls were measured as well. Intravenous exposure to DOX was performed with two doses (5 and 10 mg/kg), over a period of 4 days. Mucosal scrapes samples were collected for RNA isolation at 6, 24, 72 and 96h since initial exposure. Samples from time-matched controls (saline 10 mM Tris/NaOH buffer pH 8.5) were also quantified. This dataset is part of the TransQST collection.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Theo de Kok

PROVIDER: E-MTAB-11339 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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