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MicroRNA profiling by array of human osteosarcoma and their normal bone counterpart

ABSTRACT: Profiling array: in order to investigate the microRNAs differentially expressed between osteosarcoma and their normal bone counterpart. Specimens were kept at 4 C in RNAlater for up to 1 week, then stored at 80 C. When formal pathologic interpretation of histology from other portions of the biopsy specimen rendered a diagnosis of osteosarcoma, the RNA-preserving tissue specimens were banked and annotated. In preparation for these specific experiments, total RNA was extracted from banked specimens with the TRIzol reagent and method (Invitrogen). Control samples were derived from to-be-discarded bone fragments obtained from similarly consented patients undergoing debridement surgeries for acute, traumatic injuries to the long bones.

ORGANISM(S): Homo sapiens

DISEASE(S): osteosarcoma

SUBMITTER: Marco Galasso

PROVIDER: E-MTAB-1136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miRNA signatures associate with pathogenesis and progression of osteosarcoma.

Jones Kevin B KB Salah Zaidoun Z Del Mare Sara S Galasso Marco M Gaudio Eugenio E Nuovo Gerard J GJ Lovat Francesca F LeBlanc Kimberly K Palatini Jeff J Randall R Lor RL Volinia Stefano S Stein Gary S GS Croce Carlo M CM Lian Jane B JB Aqeilan Rami I RI

Cancer research 20120220 7

Osteosarcoma remains a leading cause of cancer death in adolescents. Treatment paradigms and survival rates have not improved in two decades. Driving the lack of therapeutic inroads, the molecular etiology of osteosarcoma remains elusive. MicroRNAs (miRNAs) have demonstrated far-reaching effects on the cellular biology of development and cancer. Their role in osteosarcomagenesis remains largely unexplored. Here we identify for the first time an miRNA signature reflecting the pathogenesis of oste ...[more]

PMID: 22350417

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