Effects of human RelA transgene on murine macrophage inflammatory responses

ABSTRACT: The NFκB family of transcription factors is a major regulator of the innate immune responses, and its dysregulation has been linked to several inflammatory diseases. In this study we focused on bone marrow derived macrophages from the recently described p65-DsRed/IκBα-eGFP transgenic strain, in which a human copy of RelA (p65) was introduced into the mouse genome. Confocal imaging analysis showed that the human RelA is expressed these cells and can translocate to the nucleus upon Toll-like receptor 4 activation (TLR4). RNA sequencing analysis of polysaccharide-stimulated macrophage cultures, revealed that the extra copy of human RelA impacts on gene transcription, affecting both NFκB and non-NFκB target genes, including immediate-early and late response NFκB target genes, such as Fos and C

INSTRUMENT(S): Linux, the RNeasy mini kit (Qiagen), TruSeq stranded Total RNA kit (Illumina), Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Mohammad Tauqeer Alam 

PROVIDER: E-MTAB-11461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress