Gene expression profile of Entamoeba histolytica trophozoites during growth under different iron concentrations.
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ABSTRACT: Entamoeba histolytica is the parasite causing amoebiasis, an infectious disease targeting the intestine and liver of humans. The molecules involved in amoeba adaptation to different iron conditions during the invasive process are unknown. To investigate the effects of iron availability on gene expression in E. histolytica, we determined by microarray experiments the gene expression profile of parasites grown in the presence of different iron concentrations. Conditions included low amounts of iron, iron starvation and iron starvation supplemented with hemoglobin (Hb). Genes encoding important proteins involved in iron metabolism, reported in other organism such as bacteria, were identified for the first time in E. histolytica. The influence of iron on the amount of these transcripts was confirmed by quantitative real-time PCR and their protein products were analyzed by Western blots
ORGANISM(S): Entamoeba histolytica
SUBMITTER: Nora Hernandez
PROVIDER: E-MTAB-1158 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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