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Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of human PMA-differentiated cell line THP-1 trained with DCATH-2 peptide against untreated controls after a 3-day rest followed by stimulation with medium or lipopolysaccharide

ABSTRACT: Trained innate immunity has been shown for human macrophages, i.e. a primary stimulation with specific compounds such as Beta-glucans, BCG vaccine or oxidized LDL generates an innate memory capacity that leads to an amplified pro-inflammatory reaction to various microbial components when restimulated days later. In this study, phorbol ester differentiated THP-1 cells were used as a model for human macrophages. The aim of this experiment was to map the biological processes involved in DCATH-2 peptide trained THP-1 cells versus control cells without restimulation and after restimulation with lipopolysaccharides.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Albert van Dijk

PROVIDER: E-MTAB-11601 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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