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Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-seq of H2A.Z and its histone chaperones during the cell cycle

ABSTRACT: The aim of this study was to uncover cell cycle dependent chromatin binding of H2A.Z and its histone chaperones. U2OS cells were stably transfected with Twin-Strep-tagged H2A.Z, ANP32e, or YL1 constructs, and WT cells were used as a negative/background control. U2OS cells were synchronised in G1 or G2-M phase using hydroxyurea or nocodazole, respectively, and protein-DNA complexes were isolated by affinity purification.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

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PROVIDER: E-MTAB-11617 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP136902

REPOSITORIES: biostudies-arrayexpress

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