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ScRNA-seq study of hippocampal microglia, perivascular macrophages and perivascular fibroblasts in pre-plaque APP-KI mice

ABSTRACT: Aim of this experiment was to characterize whether SPP1 released from the hippocampal perivascular space could imprint microglia transcriptional states. We performed scRNA-seq analysis on sorted CD140a+ perivascular fibroblast (PVF), CD45highCD11intCD206+ perivascular macrophage (PVM) and CD45intCD11intCX3CR1high microglial cell from dissected hippocampi of 6-month old wild type vs SPP1KO/KO vs AppNL-F mice vs AppNL-FxSpp1KO/KO mice. Cells were isolated from dissected hippocampi as previously described (Sala Frigerio et al.,2019).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Sebastiaan De Schepper

PROVIDER: E-MTAB-11918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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