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ScRNA-seq of stromal and immune cells in WT and TGFBR2 KO mice

ABSTRACT: Single-cell RNA-seq in mice with subcutaneous tumors was used to determine if TGFβR2 signaling in DPT+ universal fibroblasts drives LRRC15+ myofibroblast differentiation using DptIresCreERT2Tgfbr2fl/fl and WT control mice.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Soren Muller

PROVIDER: E-MTAB-12028 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ScRNA-seq of stromal cells in WT and LRRC15 DTR mice

Project description:KPR tumors were subcutaneously implanted in Lrrc15DTRGFPwt/wt (DTR–) or Lrrc15DTRGFPwt/ki (DTR+) mice and DT treatment was initiated in both DTR– and DTR+ mice when tumors reached a mean volume of 100-200mm3. scRNAseq of CD24-CD45- stromal cells from a total of 40 tumor-bearing animals across both DTR– and DTR+ genotypes was carried out at four different time points including 10 days post tumor implantation and immediately prior to initiation of DT treatment (IOT) (referred to as day 0) and on days 7, 14, and 21 post IOT.

2022-08-17 | E-MTAB-12036 | biostudies-arrayexpress

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Transcription profiling of human A549 induced tumours in mice treated with PCI-5002, PCI-5003, or control vehicle

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