Unknown,Transcriptomics,Genomics,Proteomics

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Characteristics of the transcriptome of a nurse cell harboring muscle larva of Trichinella spiralis


ABSTRACT: Muscle larva of a parasitic nematode Trichinella spp. lives a portion of muscle fiber transformed to a nurse cell (NC). The NC is formed from miss-differentiated muscle satellite cells which have fused with a parasite-invaded degenerating myofiber. Though originating from muscle cells, the NC is of non-muscular type.The NC is multinuclear and hypertrophic. Molecular mechanism of the NC development remains largely unknown. The microarray project was aimed at characterization of the NC transcriptome. The NCs were isolated by enzymatic digestion from infected mouse striated muscles. Murine C2C12 myogenic cell line (ATCC) was cultured in vitro. Differentiation of myoblasts into myotubes was carried out for 6 days in a high-glucose DMEM supplemented with 2% heat-inactivated horse serum, on the plates covered with laminin and collagen IV.The NC transcriptome was referred to the transcriptomes of C2C12 myoblasts and C2C12 myotubes in two sets of camparative expression microarray hybridization experiments, NC vs myoblasts and NC vs myotubes, respectively.

ORGANISM(S): Mus musculus

SUBMITTER: Magdalena Dabrowska 

PROVIDER: E-MTAB-12042 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Nurse cell of Trichinella spp. as a model of long-term cell cycle arrest.

Dabrowska Magdalena M   Skoneczny Marek M   Zielinski Zbigniew Z   Rode Wojciech W  

Cell cycle (Georgetown, Tex.) 20080511 14


Nurse cell (NC), formed from skeletal muscle cells upon infection with parasitic nematode trichina, presents a rare system of long-term suspension in the cell cycle. Signaling pathways and general biological functions of Trichinella spiralis NC, inferred from network analysis of competitive expression microarray data (NC vs. C2C12 myoblasts and myotubes), performed in Ingenuity Pathways Analysis (IPA) software and confirmed by Real-Time PCR, are presented. Assuming 4N DNA content in NC nuclei, i  ...[more]

Publication: 1/3

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