Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-seq of livers from glucagon receptor knock-out (GcgrKO) mice compared to wildtype littermates

ABSTRACT: Glucagon is a key regulator of glucose homeostasis, amino acid catabolism, and lipid metabolism. Glucagon receptor knock-out (GcgrKO) mice have slightly reduced blood glucose levels whereas plasma levels of amino acids are vastly increased reflecting disruption of hepatic amino acid catabolism. To dissect the molecular mechanisms underlying this effect, RNA sequencing of livers from male GcgrKO mice and wild-type littermates were performed. The mice were 10 weeks of age and were subjected to a short-term fast of 4 h before anesthesia with 2.5% isoflurane.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER:

PROVIDER: E-MTAB-12060 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-12040

REPOSITORIES: biostudies-arrayexpress

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Publications

Glucagon acutely regulates hepatic amino acid catabolism and the effect may be disturbed by steatosis.

Winther-Sørensen Marie M Galsgaard Katrine D KD Santos Alberto A Trammell Samuel A J SAJ Sulek Karolina K Kuhre Rune E RE Pedersen Jens J Andersen Daniel B DB Hassing Anna S AS Dall Morten M Treebak Jonas T JT Gillum Matthew P MP Torekov Signe S SS Windeløv Johanne A JA Hunt Jenna E JE Kjeldsen Sasha A S SAS Jepsen Sara L SL Vasilopoulou Catherine G CG Knop Filip K FK Ørskov Cathrine C Werge Mikkel P MP Bisgaard Hanne Cathrine HC Eriksen Peter Lykke PL Vilstrup Hendrik H Gluud Lise Lotte LL Holst Jens J JJ Wewer Albrechtsen Nicolai J NJ

Molecular metabolism 20200913

<h4>Objective</h4>Glucagon is well known to regulate blood glucose but may be equally important for amino acid metabolism. Plasma levels of amino acids are regulated by glucagon-dependent mechanism(s), while amino acids stimulate glucagon secretion from alpha cells, completing the recently described liver-alpha cell axis. The mechanisms underlying the cycle and the possible impact of hepatic steatosis are unclear.<h4>Methods</h4>We assessed amino acid clearance in vivo in mice treated with a glu ...[more]

PMID: 32937194

Dataset Information

RNA-seq of livers from glucagon receptor knock-out (GcgrKO) mice compared to wildtype littermates

Publications

Glucagon acutely regulates hepatic amino acid catabolism and the effect may be disturbed by steatosis.

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