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Unknown,Transcriptomics,Genomics,Proteomics

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TempO-Seq of human breast cancer MCF-7 and HCC1143 cell lines under chronic normoxia and hypoxia

ABSTRACT: Comparison of luminal and basal breast cancer cells under chronic normoxia and hypoxia. Cells were plated in 96-well plates and incubated 5 days under normoxia or hypoxia after which the wells were washed once with cold PBS and lysed using TempO-Seq lysis buffer for 15 min at room temperature. Samples were stored at −80 °C before shipping to BioClavis for whole genome TempO-Seq analysis. For normoxia (NX) 21% oxygen was used and for hypoxia (HX) 1%oxygen was used. Each condition has 3 biological replicates.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Sylvia Dévédec

PROVIDER: E-MTAB-12233 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hypoxia Triggers TAZ Phosphorylation in Basal A Triple Negative Breast Cancer Cells.

Liu Qiuyu Q Stel Wanda van der WV Noord Vera E van der VEV Leegwater Hanneke H Coban Bircan B Elbertse Kim K Pruijs Joannes T M JTM Béquignon Olivier J M OJM Westen Gerard van GV Dévédec Sylvia E Le SEL Danen Erik H J EHJ

International journal of molecular sciences 20220904 17

Hypoxia and HIF signaling drive cancer progression and therapy resistance and have been demonstrated in breast cancer. To what extent breast cancer subtypes differ in their response to hypoxia has not been resolved. Here, we show that hypoxia similarly triggers HIF1 stabilization in luminal and basal A triple negative breast cancer cells and we use high throughput targeted RNA sequencing to analyze its effects on gene expression in these subtypes. We focus on regulation of YAP/TAZ/TEAD targets a ...[more]

PMID: 36077517

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