RNA-seq of E. coli BL21 (DE3) Evolved in High pH (pH = 9) Terrific Broth Media
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ABSTRACT: Bulk RNA-sequencing was performed on E. coli BL21 (DE3) evolved at 25°C in pH 9 terrific broth media buffered with Tris-HCl (pH 9). The evolved E. coli was characterised and compared to the parent strain during protein expression; the strains were actively grown and compared for gene expression at pH 7 and pH 9 in terrific broth media.
INSTRUMENT(S): NextSeq 500
ORGANISM(S): Escherichia coli BL21(DE3)
SUBMITTER: Suraj Mital
PROVIDER: E-MTAB-12337 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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